By W. N. Aldridge (eds.)
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Extra info for A Symposium on Mechanisms of Toxicity
A few days after the meeting the results of the experiment with the tail-less rats were known. It appeared that following a suitable dose of soman eight normal rats produced an average hypothermia of 5·53° ± O·l8°C whereas the eight rats which had their tails amputated 5 days previously showed a hypothermia of 4·01° ± O·l4°C. The time of minimum temperature was exactly equal in both groups. ) REFERENCES ARIENS, A. , WOLTHUIS, 0. L. & VAN BENTHEM, R. M. J. (1969). , COSTINESCU, H. & TICHESCU, G.
10. Separation of radioactive compounds obtained after denaturation of the inhibited enzyme. A mixture (vol. /nmol phosphate), KCl (0·1 M), imidazole-HCl buffer, pH 7·2 (0·01 M), EDTA (0·1 mM) was incubated for 30 min at 37°C; enzymatic activity was completely inhibited. The solution was added to a column of Sephadex G-50 (0·8 x 18 em) previously equilibrated with NH 4 HC0 3 (pH 8·0; 0·05 M) and 0·1 mM EDTA. The column was eluted with the same buffer and the fractions containing the protein (between 2·0 and 4·0 ml) were combined and heated for 3 min at 100°C.
F. (1969). Biochem. , 18, 2559 TIPTON, K. F. & SPIRES, I. P. C. (1968). Biochem. , 17,2137 YouDIM, M. B. , CoLLINS, G. G. S. & SANDLER, M. (1969). , 223, 626 YoUDIM, M. B. H. & SouRKES, T. L. (1966). Can. J. , 44, 1397 ZELLER, E. A. (1951). In The Enzymes, vol. 2, Part 1, p. 536, ed. Sumner, J. B. , New York: Academic Press ZELLER, E. , BLANKSMA, L. , BURKARD, w. , PACHA, w. L. & LAZANAS, J. C. (1959). Ann. Y. Acad. , 80, 583. SOME NEW EFFECTS OF ANTICHOLINESTERASES IN THE WHOLE ANIMAL, WITH SPECIAL EMPHASIS ON THE HYPOTHERMIA INDUCING ACTION IN THE RAT E.
A Symposium on Mechanisms of Toxicity by W. N. Aldridge (eds.)