By Saul L. Neidleman (ed.), Allen I. Laskin (ed.)
Meant for researchers in utilized microbiology and environmental engineers, this e-book covers such issues as environmental overview of biotechnological methods and microbial alterations of haloaromatic and haloaliphatic compounds.
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Extra info for Advances in Applied Microbiology, Vol. 35
K. LONSANE AND M. V. , 1961), or nutrient sporulation broth containing 8 g/liter DIFCO nutrient broth with 7 x M CaC12, 5 X M MnC12, and 1 x M MgClz (Tobey and Yousten, 1976). No noticeable effect on the production of the enzyme was reported by Feniksova et al. (1960) with the use of tap water or buffer as the moistening agent. In addition, the use of phosphate buffer was reported to complicate the subsequent purification of the enzyme because of the introduction of the salts in the system in the form of buffers.
Some of the vital nutrients necessary for optimum growth and product formation processes may also be present in WB at suboptimal levels. Hence, the supplementation of WB with other solid and/or water-soluble nutrients was found to lead to enhanced product formation in SSF processes (Kumar and Lonsane, 1987a,b). The various solid and water-soluble supplementary nutrients used along with WB for improving enzyme yield are listed in Tables IV and V, respectively. The supplementation of WB with other solid substrates was found to increase the enzyme yield in general.
A highly thermostable enzyme is produced on an industrial scale by SmF using this culture. It would be of industrial value to isolate strains of this species with the capability to produce highly thermostable a-amylase using the SSF technique. 3. The bacterial a-amylases available in the international market have a narrow pH range for enzyme activity and are largely inactivated when used in more acidic or alkaline pH ranges. The production under SSF conditions of an enzyme with a broad optimum for pH in acidic, neutral, and alkaline ranges was reported by Ramesh and Lonsane (1987b),but the yield was too low.
Advances in Applied Microbiology, Vol. 35 by Saul L. Neidleman (ed.), Allen I. Laskin (ed.)